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No wash steps
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Adherent and non-adherent cell types
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Higher signal intensity
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Improved data quality
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Compatible with more targets
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introduction
The FLIPR Calcium 3 Assay Kit provides a universal method for detecting changes in intracellular calcium concentration in a simple and reliable homogeneous assay format. Specifically, this fast and simple fluorescence-based assay yields maximum signal intensity, thereby allowing the researcher to accurately and easily detect intercellular calcium fluxes across a broad spectrum of targets. Further, the FLIPR Calcium 3 Assay Kit is designed to produce superior results from chemokine and small peptide targets, as well as transient, endogenous, orphan and low-expression receptors that have been difficult to assay with standard assay formats.
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FLIPR assays for GPCRS
Calcium mobilization assays for screening GPCR targets are preferred techniques in drug discovery because they generate high quality leads. Molecular Devices’ FLIPR system is the method of choice among pharmaceutical and biotechnology companies for screening calcium flux assays in high throughput. Molecular Devices’ FlexStation is also commonly used in assay development, target validation and lower throughput applications.
optimized assays
To simplify assay development and optimize performance on both FLIPR and FlexStation, Molecular Devices has developed a series of no-wash assay kits. The combination of proprietary chemistry and a no-wash protocol causes less cellular disruption than traditional methods to generate consistent results and strong signals. FLIPR Assay Kits provide the easiest way to get the best performance from your FLIPR or FlexStation system.
patented technology
FLIPR Assay Kits from Molecular Devices employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology (U.S. patent number 6,420,183, EPO patent number 0 906 572) is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits.
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universal format
rapid assay development
The streamlined, homogeneous format makes the FLIPR Calcium 3 Assay Kit less labor intensive to run. With the mix-and-read procedure, the cells can be incubated with the reagents and are stable for up to several hours. Rapid analysis of the cells can be followed with detection on a FLIPR system or FlexStation microplate reader.
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increased throughput
The FLIPR Calcium 3 Assay Kit increases throughput by eliminating cumbersome wash steps. Less preparation time is required for wash buffers and wash calibrations. This successfully eliminates causes for variability in the data, resulting in fewer false positives and negatives. The kit is designed to work at room temperature, which is conducive to automation using stackers or robots. Larger unit volume packaging in the Express kit format minimizes reagent bottle and liquid handling.
reduced assy failures
Cell detachment, lowered cell competency and incomplete dye washing are common problems that are easily avoided by using the FLIPR Calcium 3 Assay Kit. In addition, the elimination of potential dispensing and washing errors, or associated equipment failures, further insures the integrity of screening operations.
Ordering Information
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Product
Number
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R8091
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R8090 |
R8108
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Product
Name
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FLIPR Calcium 3 Assay Explorer Kit
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FLIPR Calcium 3 Assay Bulk Kit
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FLIPR Calcium 3 Assay Express Kit
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Unit Size
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1 Kit provides sufficient reagent for 10 plates (96- or 384-well):
(10) 1 mL reagent vials
(1) buffer bottle
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1 Kit provides sufficient reagent for 100 plates (96- or 384-well):
(10) 10 mL reagent vials
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1 Kit provides sufficient reagent for 100 plates (96- or 384-well):
(2) 50 mL reagent vials
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